Transgenic Animal Technology

Author: Carl A. Pinkert
Publisher: Academic Press
ISBN: 0323137830
Size: 58.49 MB
Format: PDF
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Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions, as well as to make practical applications to production agriculture. This well-illustrated handbook covers the technical aspects of gene transfer in animals--from molecular methods to whole animal considerations--for the important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. Contains a comprehensive collection of transgenic animal and gene transfer methods Discusses background and introduction to techniques and animal systems Teaches practical step-by-step protocols Describes mouse model systems Covers laboratory and domestic animal species Illustrated with diagrams and photographs Includes useful tables and charts

Transgenic Animal Technology

Author: Carl A. Pinkert
Publisher: Newnes
ISBN: 0124095364
Size: 61.10 MB
Format: PDF, Docs
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Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer — from molecular methods to whole animal considerations — for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. Contains a comprehensive collection of transgenic animal and gene transfer methods Discusses background and introduction to techniques and animal systems Teaches practical step-by-step protocols Fully revised with updates to reflect state-of-the-art technology and associated changes to date

Biomedical Technology And Devices Handbook

Author: George Zouridakis
Publisher: CRC Press
ISBN: 9780849311406
Size: 67.52 MB
Format: PDF, Docs
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Concise yet comprehensive, the Biomedical Technology and Devices Handbook illuminates the equipment, devices, and techniques used in modern medicine to diagnose, treat, and monitor human illnesses. With topics ranging from the basic procedures like blood pressure measurement to cutting-edge imaging equipment, biological tests, and genetic engineering, this book is organized to navigate smoothly from simple procedures and concepts to the more sophisticated and complex ones. Each section contains a description of the technique, its technical considerations, and its use according to its applications and relevant body systems. The book includes references to relevant Web sites, protocols, problems, and solutions.

Haschek And Rousseaux S Handbook Of Toxicologic Pathology

Author: Wanda M. Haschek
Publisher: Academic Press
ISBN: 0124157653
Size: 53.29 MB
Format: PDF, Mobi
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Haschek and Rousseaux's Handbook of Toxicologic Pathology is a key reference on the integration of structure and functional changes in tissues associated with the response to pharmaceuticals, chemicals and biologics. The 3e has been expanded by a full volume, and covers aspects of safety assessment not discussed in the 2e. Completely revised with many new chapters, it remains the most authoritative reference on toxicologic pathology for scientists and researchers studying and making decisions on drugs, biologics, medical devices and other chemicals, including agrochemicals and environmental contaminants. New topics include safety assessment, the drug life cycle, risk assessment, communication and management, carcinogenicity assessment, pharmacology and pharmacokinetics, biomarkers in toxicologic pathology, quality assurance, peer review, agrochemicals, nanotechnology, food and toxicologic pathology, the environment and toxicologic pathology and more. Provides new chapters and in-depth discussion of timely topics in the area of toxicologic pathology and broadens the scope of the audience to include toxicologists and pathologists working in a variety of settings Offers high-quality and trusted content in a multi-contributed work written by leading international authorities in all areas of toxicologic pathology Features hundreds of full color images in both the print and electronic versions of the book to highlight difficult concepts with clear illustrations

Laboratory Animal Medicine

Author: James G. Fox
Publisher: Academic Press
ISBN: 9780080535333
Size: 80.25 MB
Format: PDF, Docs
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A volume in the American College of Laboratory Animal Medicine series, this second edition has over 40% new material, including the addition of six new topics and many others that are completely rewritten. The book comprehensively covers the biological and disease aspects of laboratory animal medicine while examining other aspects such as the biohazards associated with the use of animal experimentation and factors complicating the bioethics of animal research.

Advanced Protocols For Animal Transgenesis

Author: Shirley Pease
Publisher: Springer
ISBN: 9783642207914
Size: 58.53 MB
Format: PDF, ePub
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This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections. Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.

Harkness And Wagner S Biology And Medicine Of Rabbits And Rodents

Author: John E. Harkness
Publisher: John Wiley & Sons
ISBN: 0813815312
Size: 76.41 MB
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"Harkness and Wagner's Biology and Medicine of Rabbits and Rodents, Fifth Edition" is a practical reference in small mammal husbandry and health, encompassing the fields of laboratory animal medicine and pet practice. Part of ACLAM's series of laboratory animal books, this text offers concise but complete coverage on rabbits and the most common rodent species, with an emphasis on biology, clinical procedures, clinical signs, and diseases and conditions. By providing useful, accessible assessment and diagnostic information, "Harkness and Wagner's Biology and Medicine of Rabbits and Rodents" aids the practitioner in diagnosing and treating conditions in small mammals. Logically organized for easy reference, the book begins with general husbandry and procedures, followed by specific disease information. "Harkness and Wagner's" "Biology and Medicine of Rabbits and Rodents" is essential reading for clinical practitioners, laboratory animal personnel, and veterinary technicians. Key features: Concise but complete coverage of small mammal husbandry and health Encompasses the fields of laboratory animal medicine, production medicine, and pet practice Logically organized for easy reference Provides assessment and diagnostic information to aid in quick diagnosis and treatment Includes clinical photographs and case studies Covers rabbits, guinea pigs, chinchillas, hamsters, gerbils, mice, and rats

The Laboratory Mouse

Author: Hans Hedrich
Publisher: Academic Press
ISBN: 012382009X
Size: 15.39 MB
Format: PDF, Docs
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The Laboratory Mouse, Second Edition is a comprehensive book written by international experts. With inclusions of the newly revised European standards on laboratory animals, this will be the most current, global authority on the care of mice in laboratory research. This well-illustrated edition offers new and updated chapters including immunology, viruses and parasites, behavior, enrichment and care standards of laboratory mice across the life sciences, medical and veterinary fields. Features four-color illustrations with complete instruction on mouse surgery, anatomy, behavior and care of the mouse in laboratory research Offers additional chapters on new mouse strains, phenotyping of strains, bacteria and parasites, and immunology Includes the newly revised EU standards on care, as well as, comparisons to standards and regulations in the US and other countries

Mammalian And Avian Transgenesis New Approaches

Author: Shirley Pease
Publisher: Springer Science & Business Media
ISBN: 9783540284895
Size: 47.79 MB
Format: PDF, ePub, Mobi
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Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds. The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.

Handbook Of Industrial Cell Culture

Author: Victor A. Vinci
Publisher: Springer Science & Business Media
ISBN: 1592593461
Size: 51.31 MB
Format: PDF, Mobi
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A diverse team of researchers, technologists, and engineers describe, in simple and practical language, the major current and evolving technologies for improving the biocatalytic capabilities of mammalian, microbial, and plant cells. The authors present state-of-the-art techniques, proven methods, and strategies for industrial screening, cultivation, and scale-up of these cells, and describe their biotech and industrial uses. Special emphasis is given to the solving critical issues encountered during the discovery of new drugs, process development, and the manufacture of new and existing compounds. Other topics include recombinant protein expression, bioinformatics, high throughput screening, analytical tools in biotechnology, DNA shuffling, and genomics discovery.