Tissue Culture

Author: Paul F. Jr. Kruse
Publisher: Elsevier
ISBN: 0323142079
Size: 70.52 MB
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Tissue Culture: Methods and Applications presents an overview of the procedures for working with cells in culture and for using them in a wide variety of scientific disciplines. The book discusses primary tissue dissociation; the preparation of primary cultures; cell harvesting; and replicate culture methods. The text also describes protocols on single cell isolations and cloning; perfusion and mass culture techniques; cell propagation on miscellaneous culture supports; and the evaluation of culture dynamics. The recent techniques facilitating microscopic observation of cells; cell hybridization; and virus propagation and assay are also encompassed. The book further tackles the production of hormones and intercellular substances; the diagnosis and understanding of disease; as well as quality control measures. Scientists and professionals interested in methodology per se will find the book invaluable.

Plant Tissue Culture

Author: Gerard Meurant
Publisher: Elsevier
ISBN: 0323139434
Size: 17.30 MB
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Plant Tissue Culture: Methods and Applications in Agriculture contains the proceedings of a symposium based on the UNESCO training course on Plant Tissue Culture: Methods and Applications in Agriculture, sponsored by UNESCO and held in Campinas, Sao Paulo, Brazil, on November 8-22, 1978. This book contains two major sections encompassing plant tissue culture: Part A, which focuses on methodology, and Part B, which emphasizes the applications. The first chapters present the requirements for a tissue culture facility, and then describe nutrition, media, and characteristics of cultured plant cells and their growth and behavior in vitro, particularly with reference to embryogenesis and organogenesis. Discussions on protoplasts, mutagenesis and in vitro selection, meristem culture, freeze preservation, and cytogenetic techniques complete Part A. In Part B, androgenesis, in vitro fertilization, and embryo culture are discussed. Some chapters follow on the application of in vitro methodology to selected crops. The final chapter deals with the potential of tissue culture in the biosynthesis of secondary products. This text will prove useful to those who must thoroughly plan their research in tackling problems in agriculture that are amenable to the tissue culture approach.

Plant Tissue Culture

Author: Trevor A. Thorpe
Publisher: New York ; Toronto : Academic Press
ISBN: 9780126906806
Size: 11.49 MB
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Requirements for a tissue culture facility. Nutrition, media, and characteristics of plant cell and tissue cultures. Growth and behavior of cell cultures: embryogenesis and organogenesis. Isolation, fusion, and culture of plant protoplasts. Mutagenesis and in vitro selection. Meristem culture and cryopreservation - methods and applications. Cytogenetic techniques. Production of isogenic lines: basic technical aspects of androgenesis. In vitro fertilization and embryo culture. In vitro methods applied to rice. In vitro methods applied to sugar cane improvement. In vitro methods applied to coffee. In vitro methods applied to forest trees. Biosynthesis of secondary products in vitro.

Practical Tissue Culture Applications

Author: Karl Maramorosch
Publisher: Elsevier
ISBN: 0323150179
Size: 67.30 MB
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Practical Tissue Culture Applications contains the proceedings of a conference held at the International Laboratory for Research on Animal Diseases in Nairobi, Kenya, August 24-29, 1978. This book aims to describe some of the more important practical applications of in vitro techniques in a simple, easily understandable manner. Organized into three sections, with a total of 27 chapters, this book provides critical reviews, describes various techniques, and presents complete step-by-step methodology. It emphasizes applications pertaining to the health and economy in developing nations. In particular, this book discusses the pitfalls in preparing general purpose culture media, balanced salt solutions, and the procedures followed in the development of modern in vitro techniques. It also describes techniques for cultivation of vertebrate cells and organs; plant tissue culture and its numerous applications; and electron microscopy of cultured cell. This book explains as well virus isolation and identification in cell cultures, mass production of cells for vaccines, and use of cultured cells for drug evaluation. The applications of in vitro techniques to parasitology are explored in numerous chapters of this book. Considering the potential benefit of application of in vitro techniques, this reference material will be of interest both in developed and developing countries.

Modern Applications Of Plant Biotechnology In Pharmaceutical Sciences

Author: Saurabh Bhatia
Publisher: Academic Press
ISBN: 0128024984
Size: 38.19 MB
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Modern Applications of Plant Biotechnology in Pharmaceutical Sciences explores advanced techniques in plant biotechnology, their applications to pharmaceutical sciences, and how these methods can lead to more effective, safe, and affordable drugs. The book covers modern approaches in a practical, step-by-step manner, and includes illustrations, examples, and case studies to enhance understanding. Key topics include plant-made pharmaceuticals, classical and non-classical techniques for secondary metabolite production in plant cell culture and their relevance to pharmaceutical science, edible vaccines, novel delivery systems for plant-based products, international industry regulatory guidelines, and more. Readers will find the book to be a comprehensive and valuable resource for the study of modern plant biotechnology approaches and their pharmaceutical applications. Builds upon the basic concepts of cell and plant tissue culture and recombinant DNA technology to better illustrate the modern and potential applications of plant biotechnology to the pharmaceutical sciences Provides detailed yet practical coverage of complex techniques, such as micropropogation, gene transfer, and biosynthesis Examines critical issues of international importance and offers real-life examples and potential solutions

Cells And Tissues In Culture

Author: E. N. Willmer
Publisher: Elsevier
ISBN: 1483223000
Size: 41.54 MB
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Cells and Tissues in Culture: Methods, Biology and Physiology, Volume 1 covers the general fields of tissue culture, including an evaluation of its technique, effects, and contributions to biology. This book focuses on the three methods of culture—tissue culture, cell culture, and organ culture.Other topics include the design of complete synthetic media, possible evolution of the cell types, and energy relationships in growing and stationary cells. The RNA synthesis in cell cultures, culture of amphibian embryonic anlage, action of corticosteroids and adrenaline, and effects of parathyroid hormone on bone are also elaborated. This volume is recommended for biologists and specialists interested in the culture of cells and tissues.

Plant Tissue Culture Engineering

Author: S. Dutta Gupta
Publisher: Springer Science & Business Media
ISBN: 9781402036941
Size: 29.51 MB
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It is my privilege to contribute the foreword for this unique volume entitled: “Plant Tissue Culture Engineering,” edited by S. Dutta Gupta and Y. Ibaraki. While there have been a number of volumes published regarding the basic methods and applications of plant tissue and cell culture technologies, and even considerable attention provided to bioreactor design, relatively little attention has been afforded to the engineering principles that have emerged as critical contributions to the commercial applications of plant biotechnologies. This volume, “Plant Tissue Culture Engineering,” signals a turning point: the recognition that this specialized field of plant science must be integrated with engineering principles in order to develop efficient, cost effective, and large scale applications of these technologies. I am most impressed with the organization of this volume, and the extensive list of chapters contributed by expert authors from around the world who are leading the emergence of this interdisciplinary enterprise. The editors are to be commended for their skilful crafting of this important volume. The first two parts provide the basic information that is relevant to the field as a whole, the following two parts elaborate on these principles, and the last part elaborates on specific technologies or applications.

Plant Tissue Culture And Its Agricultural Applications

Author: Lyndsey A. Withers
Publisher: Butterworth-Heinemann
ISBN: 1483192059
Size: 79.11 MB
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Plant Tissue Culture and Its Agricultural Applications presents the proceedings of the 41st University of Nottingham Easter School in Agricultural Science held in England. The sessions covered in this volume reflect the revolution of tissue culture and its role in the propagation of elite plant material and the development of improved genotypes. This book is organized into four main sections. The first section chronicles the revolution of the plant tissue culture. This includes papers on clonal propagation, morphogenesis, germplasm storage, plant health, and genetic improvement. The core of this volume is covered by the introductory and the final chapters which interrelate the different subjects areas covered by the proceedings and provide a realistic assessment of future research required for the plant tissue culture revolution to come to fruition. This book will be useful to readers interested in understanding the history, evolution, and future of plant tissue culture and its applications in the agricultural sector.

Plant Cell And Tissue Culture A Tool In Biotechnology

Author: Karl-Hermann Neumann
Publisher: Springer Science & Business Media
ISBN: 9783540938835
Size: 11.12 MB
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This book provides a general introduction as well as a selected survey of key advances in the fascinating field of plant cell and tissue culture as a tool in biotechnology. After a detailed description of the various basic techniques employed in leading laboratories worldwide, follows an extended account of important applications in, for example, plant propagation, secondary metabolite production and gene technology. Additionally, some chapters are devoted to historical developments in this domain, metabolic aspects, nutrition, growth regulators, differentiation and the development of culture systems. The book will prove useful to both newcomers and specialists, and even “old hands” in tissue culture should find some challenging ideas to think about.

Culture Of Animal Cells

Author: R. Ian Freshney
Publisher: John Wiley & Sons
ISBN: 1118873378
Size: 20.72 MB
Format: PDF, Mobi
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Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made – particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols. This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians.