Protein Engineering

Author: Raghupathy Sarma
Publisher: Elsevier
ISBN: 0323150306
Size: 48.13 MB
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Protein Engineering: Applications in Science, Medicine, and Industry deals with the scientific, medical, and industrial applications of protein engineering. Topics range from protein structure and design to mutant analysis and complex systems. Applications such as production of novel antibiotics, genetic transformation of plants, and genetic engineering of bioinsecticides are described. This book is comprised of 25 chapters and begins with an overview of trends and developments in protein chemistry and their relevance to protein engineering, followed by a discussion on protein sequence data banks. Subsequent chapters explore the design and construction of biologically active peptides, including hormones; structural and functional analysis of thermophile proteins; the conformation of diphtheria toxin; and applications of surface-simulation synthesis in protein molecular recognition. The use of oligonucleotide-directed site-specific mutagenesis in functional analysis of the signal peptide for protein secretion is also considered. The results of studies on the mechanism of membrane fusion are presented. This monograph will serve as a useful guide for those who are already working on protein engineering and those who are about to start research in this field.

Protein Engineering For Industrial Biotechnology

Author: Lilia Alberghina
Publisher: CRC Press
ISBN: 1482283484
Size: 61.84 MB
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Protein engineering has proved to be one of the more fruitful technological approaches in biotechnology, being both very powerful and able to generate valuable intellectual property. This book aims to present examples in which the application of protein engineering has successfully solved problems arising in industrial biotechnology. There is a section on its use to enhance purification of recombinant proteins. The use of protein engineering to modify the activity or the stability of industrial enzymes from lipases to proteases, from carboxypeptidases to glucanases and glucosidases, and from pectin modifying enzymes to enzymes able to degrade recalcitrant compounds is extensively covered. It is shown how areas as diverse as agrofood technology, fine chemistry, detergents, bioremediation and biosensors receive significant contributions from protein and solvent engineering. The application of protein engineering to health care is also covered, from the development of new vaccines to new potential therapeutic proteins. A specific notation is given to protein engineering in the development of target molecules for drug discovery. International in scope, the many contributions are drawn from academia and industry. The text should be of interest to students and researchers in industrial biotechnology as well as to everybody interested in basic research in protein structure, molecular genetics, bio-organic chemistry, biochemistry, agrobiotechnology, pharmaceutical sciences and medicine.

Proteins Form And Function

Author: R.A. Bradshaw
Publisher: Elsevier
ISBN: 0080983936
Size: 44.27 MB
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Proteins are of fundamental importance in all aspects of cell structure and function. The study of proteins has always formed a central part of biochemistry, and recent expansion in the range and sophistication of available techniques has provided a wealth of new information. The current methods and approaches used to gain a better understanding of the structure of proteins are described within this book, while other articles focus on the role of proteins within the cell. Most of the articles have appeared previously in the monthly review journal Trends in Biochemical Sciences (TIBS), with a few commissioned specifically for this collection, which should appeal to students, lecturers and researchers interested in the form and function of proteins.

Medicinal Protein Engineering

Author: Yury E. Khudyakov
Publisher: CRC Press
ISBN: 9781420007305
Size: 60.72 MB
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An All-Inclusive Review of the Achievements and Trends in the Fast-Growing Protein Engineering Field From humble beginnings like making fire for mere survival, engineering now steadfastly penetrates all aspects of our lives and even life itself at the molecular level. Protein engineering is a molecular biological discipline focused on designing and constructing novel proteins with desired properties. The currently limited understanding of the relationship between protein structure and function greatly hinders rational protein design. However, despite great challenges, protein engineering has become a major molecular discipline with a large array of successful applications to many complex medicinal problems. Medicinal Protein Engineering sheds light on this largely unchartered field, covering major strategies for engineering of proteins with predetermined biological properties. It discusses computational approaches to protein design and experimental approaches to protein construction. This volume also explores the tight connection between protein and genetic engineering. It moves researchers beyond experimental protein construction and theoretical protein design to the medicinal applications of engineered proteins. Examines Medicinal Applications of Protein Engineering for the Diagnosis, Treatment, and Prevention of Diseases Focusing on the application of protein engineering to medicine, this seminal work outlines the appropriate techniques for studying protein properties and building mathematical engineering models of novel vaccines, diagnostic reagents, and therapeutic treatments. As a truly comprehensive assessment of the medical protein engineering research available and its future implications for disease control and prevention, this is an indispensable reference for biological researchers in this groundbreaking field.

Molecular Electronics And Molecular Electronic Devices

Author: Kristof Sienicki
Publisher: CRC Press
ISBN: 9780849380624
Size: 69.75 MB
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Molecular Electronics and Molecular Electronic Devices is a book that provides a comprehensive review of current problems and information regarding aspects of molecular electronics and molecular electronic devices. Experimental and theoretical aspects of molecular electronics and molecular electronic devices are reviewed by distinguished researchers working in chemistry, physics, computer science, and various areas of biology. These books will be an excellent reference for physicists, chemists, electronics engineers and researchers interested in molecular electronics and molecular electronic devices.

Protein Engineering Handbook

Author: Stefan Lutz
Publisher: John Wiley & Sons
ISBN: 3527666982
Size: 19.29 MB
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This introduction collects 17 innovative approaches to engineer novel and improved proteins for diverse applications in biotechnology, chemistry, bioanalytics and medicine. As such, key developments covered in this reference and handbook include de novo enzyme design, cofactor design and metalloenzymes, extremophile proteins, and chemically resistant proteins for industrial processes. The editors integrate academic innovations and industrial applications so as to arrive at a balanced view of this multi-faceted topic. Throughout, the content is chosen to complement and extend the previously published two-volume handbook by the same editors, resulting in a superb overview of this burgeoning field.

Protein Engineering And Design

Author: Sheldon J. Park
Publisher: CRC Press
ISBN: 9781420076592
Size: 75.46 MB
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Experimental protein engineering and computational protein design are broad but complementary strategies for developing proteins with altered or novel structural properties and biological functions. By describing cutting-edge advances in both of these fields, Protein Engineering and Design aims to cultivate a synergistic approach to protein science. Experimental Protein Engineering The first half of the book discusses experimental approaches to protein engineering and starts by describing several high-throughput screening platforms for protein engineering. Key techniques used for diversity generation are also discussed. The next few chapters present examples of therapeutics, enzymes, biomaterials, and other proteins that have been engineered by rational or combinatorial approaches. The section finishes with a chapter on the use of non-natural amino acids in protein engineering. Computational Protein Design The second half of the book introduces computational protein design, beginning with a chapter on computational and informatics algorithms used in protein engineering. Core components of computational protein design are then discussed in detail, and examples of heuristic protein design are provided. Subsequent chapters present examples of how computational design has played a critical role in advancing the field of protein engineering. Concluding with a chapter outlining current challenges in the field, this book makes computational protein design and diversity-oriented protein engineering widely accessible to a broad audience in academia and industry alike.

Protein Engineering

Author: Peter C. E. Moody
Publisher: Oxford University Press, USA
ISBN: 9780199631940
Size: 79.74 MB
Format: PDF
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This is a clear, up-to-date review of the scientific techniques used to alter the properties of proteins. It provides a wealth of in-depth information in an accessible format, explaining the underlying principles and their useful applications. Topics covered include the analysis of mutant proteins, structure-activity relationships, and the application of protein engineering to industrial and medical problems. The book also reviews a number of areas in which recent findings have led to new ideas that are not adequately described in existing textbooks. Two-color illustrations clarify the material and help make this an ideal source of information for undergraduates and graduates in biology and medicine.

Bionanotechnology

Author: Anil Kumar Anal
Publisher: CRC Press
ISBN: 1351643630
Size: 24.28 MB
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This book deals with a subject of high interest and importance in all sectors, including biomedical, food, agriculture, energy, and environment. Biological systems are essential in nanotechnology, and many new applications are being developed by mimicking the natural systems. Approaching these topics from an engineering perspective, the book offers insight on the details of nanoscale fabrication processes as well as cell biology. The basics of biology and chemistry, with a focus on how to engineer the behavior of molecules at the nanoscale, are also explored and analyzed. The aim of the text is to provide the reader with broader knowledge of biological methods for signal transduction and molecular recognitions systems and how they can be replicated in bio-sensing applications. The reader will learn the basic structures and interactions of biomacromolecules for developing biocompatible and eco-friendly devices.