Plant Molecular Biology Manual

Author: USA (Ed. ). Gelvin, S. B., Purdue University, West Lafayette, IN
Publisher: Springer
ISBN: 9401769532
Size: 60.50 MB
Format: PDF, Kindle
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Methods In Plant Molecular Biology

Author: Mary A. Schuler
Publisher: Academic Press
ISBN: 0323137652
Size: 74.65 MB
Format: PDF, ePub
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Methods in Plant Molecular Biology is a lab manual that introduces students to a diversity of molecular techniques needed for experiments with plant cells. Those included have been perfected and are now presented for the first time in a usable and teachable form. Because the manual integrates protein, RNA, and DNA techniques, it will serve students, teachers, and researchers in plant physiology, biophysics, and animal molecular biology who have no previous experience handling recombinant DNA or purified proteins. It can also be used by the established molecular biologist who wishes to utilize the powerful techniques of recombinant DNA to explore the mysteries of the plant kingdom. Eight basic experiments which can be used collectively or individually cover Recombinant Cloning and Screening in E. coli; DNA Sequencing Plant RNA Isolation and in Vitro Translations Plant DNA Isolations and Genomic DNA Southern Analysis Chloroplast Isolation and Protein Synthesis Plant Tissue Culture and Agrobacterium Transformations Experiments that have been student tested for three years Blueprints for setting up gel rigs Comprehensive course schedule outlining individual procedures to be finished in each lab segment Course can be tailored to suit the needs of the individual instructor

Plant Molecular Biology

Author: USA (Ed. ). Gelvin, S. B., Purdue University, West Lafayette, IN
Publisher: Springer
ISBN: 9401769516
Size: 69.48 MB
Format: PDF
View: 4015
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Practical Applications Of Plant Molecular Biology

Author: Robert J. Henry
Publisher: Nelson Thornes
ISBN: 9780412732201
Size: 45.61 MB
Format: PDF
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Practical Applications of Plant Molecular Biology is an important new title which covers the major techniques and how they are applied to a range of vitally important areas. Divided broadly into four sections, this book covers key subjects including the identification of plants and plant pathogens using molecular techniques, the estimation of genetic variation in plants, the use of molecular markers in plant improvement and the use of plant transformation techniques for the improvement of quality and the introduction of resistance. Also included is a comprehensive listing and description of the most frequently used techniques and a set of appendices covering useful topics of reference for the reader. All undergraduates studying plant sciences, molecular biology, biotechnology and agricultural sciences would benefit from having access to this title as would those studying for upper-level Masters courses concentrating on the disciplines covered. This book also provides an invaluable source of reference for professionals in agriculture, plant breeding, crop protection and improvement, biotechnology and molecular biology.

Molecular Biology Techniques

Author: Walt Ream
Publisher: Academic Press
ISBN: 0080536824
Size: 57.27 MB
Format: PDF, Docs
View: 1993
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This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter

Plant Molecular Biology Labfax

Author: R. R. D. Croy
Publisher: Elsevier
ISBN: 9780121983703
Size: 63.93 MB
Format: PDF, ePub, Docs
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LABFAX volumes are purpose-designed data reference books for practicing scientists. Each book presents key information for a major subject in one place and so saves hours of searching. It does not simply collect together data which are already available in catalogs, since these are often incomplete and can contain conflicting information. Rather, the authors and editors of each LABFAX volume have searched the original literature for the accurate data which they know the specialist needs. Plant Molecular Biology Labfax is a detailed compendium of essential information on plant nucleic acids, transformation and expression vectors, selectable genes and reporter genes, gene expression and PCR techniques, etc. A key feature is the Plant Gene Index, comprising comprehensive tables of plant genes published and submitted to sequence databases. Plant Molecular Biology Labfax, while specializing in molecular aspects of plant science, inevitably has numerous sections dealing with general molecular biology which complement the extensive information provided in Molecular Biology Labfax. It is therefore a worthy companion to this text.

Molecular Biology Techniques

Author: Heather Miller
Publisher: Academic Press
ISBN: 0123855454
Size: 35.55 MB
Format: PDF, Mobi
View: 464
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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions