Dna Fingerprinting In Plants

Author: Kurt Weising
Publisher: CRC Press
ISBN: 9781420040043
Size: 57.25 MB
Format: PDF
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Given the explosive development of new molecular marker techniques over the last decade, newcomers and experts alike in the field of DNA fingerprinting will find an easy-to-follow guide to the multitude of techniques available in DNA Fingerprinting in Plants: Principles, Methods, and Applications, Second Edition. Along with step-by-step annotated protocols, the authors fully discuss the technical aspects and modifications of existing techniques, the influence of reaction components and conditions, and the analysis of the results. This second edition has been completely revised to address the exponential changes in the field since the first edition, focusing on PCR-based techniques but also including more sophisticated ones. The authors include numerous case studies to illustrate applications of the methods, more than 1600 references to the literature, and descriptions of reagent formulation, equipment, and computer programs used for evaluating molecular marker data. They compare the various methods, including the costs and benefits of each, helping readers determine which is best suited to a particular application. The well-rounded, cross-referenced, and unified nature of this book makes it intrinsically easier to follow than the edited, multi-authored books currently available. It is an absolute necessity on the lab bench of anyone involved in plant research, DNA profiling, and molecular markers.

Dna Fingerprinting In Plants

Author: Kurt Weising
Publisher: CRC Press
ISBN: 9781439883327
Size: 34.54 MB
Format: PDF, Docs
View: 3804
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Given the explosive development of new molecular marker techniques over the last decade, newcomers and experts alike in the field of DNA fingerprinting will find an easy-to-follow guide to the multitude of techniques available in DNA Fingerprinting in Plants: Principles, Methods, and Applications, Second Edition. Along with step-by-step annotated protocols, the authors fully discuss the technical aspects and modifications of existing techniques, the influence of reaction components and conditions, and the analysis of the results. This second edition has been completely revised to address the exponential changes in the field since the first edition, focusing on PCR-based techniques but also including more sophisticated ones. The authors include numerous case studies to illustrate applications of the methods, more than 1600 references to the literature, and descriptions of reagent formulation, equipment, and computer programs used for evaluating molecular marker data. They compare the various methods, including the costs and benefits of each, helping readers determine which is best suited to a particular application. The well-rounded, cross-referenced, and unified nature of this book makes it intrinsically easier to follow than the edited, multi-authored books currently available. It is an absolute necessity on the lab bench of anyone involved in plant research, DNA profiling, and molecular markers.

Dna Fingerprinting Approaches And Applications

Author: Gaudenz Dolf
Publisher: Birkhäuser
ISBN: 3034873123
Size: 42.91 MB
Format: PDF, ePub
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Although DNA fingerprinting is a very young branch of molecular genetics, being barely six years old, its recent impact on science, law and politics has been dramatic. The application of DNA finger printing to forensic and legal medicine has guaranteed a high public profile for this technology, and indeed, scarcely a week goes by with out the press reporting yet another crime successfully solved by molec ular genetics. Less spectacularly, but equally importantly, DNA typing methods are steadily diffusing into an ever wider set of applications and research fields, ranging from medicine through to conservation biology. To date, two DNA fingerprinting workshops have been held in the UK, one in 1988 organised by Terry Burke at the University of Leicester, and the second in 1989 at the University of Nottingham, co-ordinated by David Parkin. In parallel with these workshops, which have provided an important focus for researchers, Bill Amos and Josephine Pemberton in Cambridge have established an informal newsletter "Fingerprint News" which is playing a major role as a forum for DNA fingerprinters. By 1989, it was clear that the field had broadened sufficiently to warrant a full international meeting. As a result, Gaudenz Dolf took on the task of organising the first, of what I hope will be many, International Symposium of DNA Fingerprinting held at Bern during Ist-3rd October 1990. The success of the meeting can be judged from the remarkable attendance, with 270 delegates from no less than 30 countries.

Botanicals

Author: Kurt Reynertson
Publisher: CRC Press
ISBN: 1466598425
Size: 53.69 MB
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The international trade in plants is growing steadily as the worldwide demand for natural and botanical raw materials increases. Customers value natural products and botanicals as "green" alternatives—safer ingredients for their families which also represent an environmentally and socially responsible choice for the planet. In order to build assurance into the sourcing of natural ingredients, R&D organizations must have valid scientific matrices to authenticate the quality of those ingredients, provide traceability, and minimize risk. An assemblage of insight from expert contributors, Botanicals: Methods and Techniques for Quality & Authenticity compiles a range of methods and techniques that can be used to help guide quality and authenticity determinations. Topics include: Metabolic profiling, authentication of botanicals by morphology, and genetic methods of botanical authentication Tools for building models for the authentication of materials How multivariate statistics can play a role in determining botanical quality and authenticity Radiocarbon and stable isotope ratio analysis and emerging stable isotope tools NMR (nuclear magnetic resonance) spectroscopy, NIR (near-infrared), and HPTLC (high-performance thin-layer chromatography) methods for analysis The use of electronic sensing instruments and applications for analysis The contributors also discuss the challenge of identifying a botanical extract or preparation on the basis of its chemical content and discuss quality issues faced by botanicals used as cosmetic ingredients. The book provides you with a range of traditional, taxonomic, and newer analytical tools to assure the quality, authenticity, and traceability of botanical raw materials for dietary supplements, cosmetics, and natural products research.

Insect Molecular Genetics

Author: Marjorie A. Hoy
Publisher: Academic Press
ISBN: 0240821319
Size: 58.12 MB
Format: PDF, ePub
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Insect Molecular Genetics, Third Edition, summarizes and synthesizes two rather disparate disciplines—entomology and molecular genetics. This volume provides an introduction to the techniques and literature of molecular genetics; defines terminology; and reviews concepts, principles, and applications of these powerful tools. The world of insect molecular genetics, once dominated by Drosophila, has become much more diverse, especially with the sequencing of multiple arthropod genomes (from spider mites to mosquitoes). This introduction includes discussion of honey bees, mosquitoes, flour beetles, silk moths, fruit flies, aphids, house flies, kissing bugs, cicadas, butterflies, tsetse flies and armyworms. This book serves as both a foundational text and a review of a rapidly growing literature. With fully revised and updated chapters, the third edition will be a valuable addition to the personal libraries of entomologists, geneticists, and molecular biologists. Up-to-date references to important review articles, websites, and seminal citations in the disciplines Well crafted and instructive illustrations integral to explaining the techniques of molecular genetics Glossary of terms to help beginners learn the vocabulary of molecular biology

Fundamentals Of Forensic Dna Typing

Author: John M. Butler
Publisher: Academic Press
ISBN: 9780080961767
Size: 56.17 MB
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Fundamentals of Forensic DNA Typing is written with a broad viewpoint. It examines the methods of current forensic DNA typing, focusing on short tandem repeats (STRs). It encompasses current forensic DNA analysis methods, as well as biology, technology and genetic interpretation. This book reviews the methods of forensic DNA testing used in the first two decades since early 1980’s, and it offers perspectives on future trends in this field, including new genetic markers and new technologies. Furthermore, it explains the process of DNA testing from collection of samples through DNA extraction, DNA quantitation, DNA amplification, and statistical interpretation. The book also discusses DNA databases, which play an important role in law enforcement investigations. In addition, there is a discussion about ethical concerns in retaining DNA profiles and the issues involved when people use a database to search for close relatives. Students of forensic DNA analysis, forensic scientists, and members of the law enforcement and legal professions who want to know more about STR typing will find this book invaluable. Includes a glossary with over 400 terms for quick reference of unfamiliar terms as well as an acronym guide to decipher the DNA dialect Continues in the style of Forensic DNA Typing, 2e, with high-profile cases addressed in D.N.A.Boxes-- "Data, Notes & Applications" sections throughout Ancillaries include: instructor manual Web site, with tailored set of 1000+ PowerPoint slides (including figures), links to online training websites and a test bank with key

Molecular Medical Microbiology

Author: Yi-Wei Tang
Publisher: Academic Press
ISBN: 0123977630
Size: 42.38 MB
Format: PDF
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The molecular age has brought about dramatic changes in medical microbiology, and great leaps in our understanding of the mechanisms of infectious disease. Molecular Medical Microbiology is the first book to synthesise the many new developments in both molecular and clinical research in a single comprehensive resource. This timely and authoritative three-volume work is an invaluable reference source of medical bacteriology. Comprising more than 100 chapters, organized into 17 major sections, the scope of this impressive work is wide-ranging. Written by experts in the field, chapters include cutting-edge information, and clinical overviews for each major bacterial group, in addition to the latest updates on vaccine development, molecular technology and diagnostic technology. Topics covered include bacterial structure, cell function, and genetics; mechanisms of pathogenesis and prevention; antibacterial agents; and infections ranging from gastrointestinal to urinary tract, centrtal nervous system, respiratory tract, and more. The first comprehensive and accessible reference on molecular medical microbiology Full color presentation througout In-depth discussion of individual pathogenic bacteria in a system-oriented approach Includes a clinical overview for each major bacterial group Presents the latest information on vaccine development, molecular technology, and diagnostic technology More than 100 chapters covering all major groups of bacteria Written by an international panel of authors who are experts in their respective disciplines

Tree Biotechnology

Author: Kishan Gopal Ramawat
Publisher: CRC Press
ISBN: 1466597143
Size: 18.85 MB
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Forest trees cover 30% of the earth's land surface, providing renewable fuel, wood, timber, shelter, fruits, leaves, bark, roots, and are source of medicinal products in addition to benefits such as carbon sequestration, water shed protection, and habitat for 1/3 of terrestrial species. However, the genetic analysis and breeding of trees has lagged behind that of crop plants. Therefore, systematic conservation, sustainable improvement and pragmatic utilization of trees are global priorities. This book provides comprehensive and up to date information about tree characterization, biological understanding, and improvement through biotechnological and molecular tools.

Dangerous Liaisons

Author: Norman C. Ellstrand
Publisher: JHU Press
ISBN: 9780801881909
Size: 55.75 MB
Format: PDF, Kindle
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"A rigorous and even-handed look at the science and technology surrounding crop gene flow." -- Biotechnology "A valuable evaluation and discussion of our knowledge of gene flow in plants and the implication for transgene movement into wild crop relatives." -- Bioscience